中文摘要：南瓜曲叶病毒（SqLCV）是一种危害瓜类作物的菜豆金色花叶病毒属双生病毒。其通过B型烟粉虱传播，可引起严重的叶片卷曲、叶脉结节、矮缩等症状。本研究利用SqLCV埃及分离物全长基因克隆和转录后基因沉默（PTGS）诱导抗病毒性的形成。
外文摘要：Squash leaf curl virus (SqLCV) is a bipartite begomovirus affecting squash plants. It is transmitted by whitefly Bemisia tabaci biotype B causing severe leaf curling, vein banding, and molting ending by stunting. In this study full-length genomic clone of SqLCV Egyptian isolated and posttranscriptional gene silencing (PTGS) has been induced to develop virus resistance. The Noubaria SqLCV has more than 95% homology with Jordon, Israel, Lebanon, Palestine, and Cairo isolates. Two genes fragment from SqLCV introduced in sense and antisense orientations using pFGC5049 vector to be expressed as hairpin RNA. The first fragment was 348 bp from replication associated protein gene (Rep). The second fragment was 879 bp representing the full sequence of the movement protein gene (BC1). Using real-time PCR, a silencing record of 97% has been recorded to Rep/TrAP construct; as a result it has prevented the appearance of viral symptoms in most tested plants up to two months after infection, while construct containing the BC1 gene scored a reduction in the accumulation of viral genome expression as appearing in real-time PCR results 4.6-fold giving a silencing of 79%, which had a positive effect on symptoms development in most tested plants.
作者：Taha, Omnia；Farouk, Inas；Abdallah, Abdelhadi；等
作者单位：开罗大学
期刊名称：INTERNATIONAL JOURNAL OF GENOMICS
期刊影响因子：1.83
出版年份：2016
出版刊次：2
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