中文摘要：拟南芥中一些L型凝集素类受体激酶（LecRKs）已被确定为潜在的免疫受体。然而，到目前为止，对作物中LecRKs方面的分析非常少。病毒诱导的CaLecRK-S.5基因沉默已经证实了CaLecRK-S.5在广谱抗病性中的作用。本研究结果表明，CaLecRK-S.5介导的广谱抗病性与启动调节有关。
外文摘要：In Arabidopsis, several L-type lectin receptor kinases (LecRKs) have been identified as putative immune receptors. However, to date, there have been few analyses of LecRKs in crop plants. Virus-induced gene silencing of CaLecRK-S.5 verified the role of CaLecRK-S.5 in broad-spectrum resistance. Compared with control plants, CaLecRK-S.5-silenced plants showed reduced hypersensitive response, reactive oxygen species burst, secondary metabolite production, mitogen-activated protein kinase activation, and defense-related gene expression in response to Tobacco mosaic virus pathotype P-0 (TMV-P-0) infection. Suppression of CaLecRK-S.5 expression significantly enhanced the susceptibility to Pepper mild mottle virus pathotype P-1,P-2,P-3, Xanthomonas campestris pv. vesicatoria, Phytophthora capsici, as well as TMV-P-0. Additionally, beta-aminobutyric acid treatment and a systemic acquired resistance assay revealed that CaLecRK-S.5 is involved in priming of plant immunity. Pre-treatment with beta-aminobutyric acid before viral infection restored the reduced disease resistance phenotypes shown in CaLecRK-S.5-silenced plants. Systemic acquired resistance was also abolished in CaLecRK-S.5-silenced plants. Finally, RNA sequencing analysis indicated that CaLecRK-S.5 positively regulates plant immunity at the transcriptional level. Altogether, these results suggest that CaLecRK-S.5-mediated broad-spectrum resistance is associated with the regulation of priming.
外文关键词：Broad-spectrum resistance；CaLecRK-S.5；L-type lectin receptor kinase；priming；RNA sequencing；SAR；tobamovirus
作者：Woo, Joo Yong；Jeong, Kwang Ju；Kim, Young Jin；等
作者单位：韩国大学
期刊名称：JOURNAL OF EXPERIMENTAL BOTANY
期刊影响因子：5.677
出版年份：2016
出版刊次：10
点击下载：CaLecRK-S.5是一种辣椒L型凝集素类受体激酶基因，可通过激活启动使作物具有广谱抗病性