中文摘要：RNA沉默是一种在进化上存在保守性的抗病毒机制。病毒来源的小干扰RNAs通过RNA沉默在感染病毒的植物中产生，并在寄主抗病毒防御中起着重要的作用。深度测序技术通过vsiRNAs丰度分析彻底改变了对病毒与寄主植物间交互作用的研究。然而，对于同一寄主植物不同组织vsiRNAs 丰度的比较研究很少有报道。本文对感染黄瓜绿斑驳花叶病毒（CGMMV）的葫芦属植物的叶片和果实间vsiRNAs丰度进行了全面的特征研究与比较，首次提供了对同一种寄主植物不同组织的vsiRNA丰度间高分辨率对照研究。
外文摘要：RNA silencing is an evolutionarily conserved antiviral mechanism, through which virus-derived small interfering RNAs (vsiRNAs) playing roles in host antiviral defence are produced in virus-infected plant. Deep sequencing technology has revolutionized the study on the interaction between virus and plant host through the analysis of vsiRNAs profile. However, comparison of vsiRNA profiles in different tissues from a same host plant has been rarely reported. In this study, the profiles of virus-derived small interfering RNAs (vsiRNAs) from leaves and fruits of Lagenaria siceraria plants infected with Cucumber green mottle mosaic virus (CGMMV) were comprehensively characterized and compared. Many more vsiRNAs were present in infected leaves than in fruits. vsiRNAs from both leaves and fruits were mostly 21- and 22-nt in size as previously described in other virus-infected plants. Interestingly, vsiRNAs were predominantly produced from the viral positive strand RNAs in infected leaves, whereas in infected fruits they were derived equally from the positive and negative strands. Many leaf-specific positive vsiRNAs with lengths of 21-nt (2,058) or 22-nt (3,996) were identified but only six (21-nt) and one (22-nt) positive vsiRNAs were found to be specific to fruits. vsiRNAs hotspots were only present in the 5鈥-terminal and 3鈥-terminal of viral positive strand in fruits, while multiple hotspots were identified in leaves. Differences in GC content and 5'-terminal nucleotide of vsiRNAs were also observed in the two organs. To our knowledge, this provides the first high-resolution comparison of vsiRNA profiles between different tissues of the same host plant.
外文关键词：virus-derived small RNAs；CGMMV；tissue-specific；NGS data analysis；Lagenaria siceraria
作者：Li, Junmin；Zheng, Hongying；Zhang, Chenhua；等
作者单位：浙江农业科学院
期刊名称：FRONTIERS IN MICROBIOLOGY
期刊影响因子：4.165
出版年份：2016
出版刊次：11
点击下载：感染黄瓜绿斑驳花叶病毒的葫芦属植物的叶片与果实间不同病毒来源的siRNAs丰度