中文摘要：瓜类褪绿黄化病毒（CCYV）是一种新型植物病毒，现对许多亚洲国家的瓜类作物造成了严重的经济损失。CCYV被认为仅由烟粉虱以半持久性方式传播。本文研究了CCYV的传播特征，并用激光共聚焦显微镜对介体昆虫体内的病毒进行免疫荧光标记和定位，研究结果为烟粉虱地中海隐种以半持久性方式传播CCYV提供了直接证据。
外文摘要：Cucurbit chlorotic yellows virus (CCYV) ( genus Crinivirus, family Closteroviridae) is an emerging plant virus, and is now spreading and causing severe economic losses to cucurbit crops in many Asian countries. CCYV is believed to be transmitted specifically by the sweetpotato whitefly, Bemisia tabaci, in a semipersistent manner. In the present study, we provide direct evidence for the semipersistent transmission of CCYV by Mediterranean (MED) cryptic species of B. tabaci complex. We investigated CCYV transmission characteristics, and immuno fluorescently labeled and localized the virus retention site within the vector by laser confocal microscopy. Whiteflies required >= 1 h of acquisition access period (AAP) to successfully acquire CCYV, and the proportion of RT-PCR positive whitefly individuals reached to 100% at 48 h of AAP. CCYV virons could be retained within vectors as long as 12 d, but the proportion of RT-PCR positive whiteflies dropped to 55% by 3 d. Groups of thirty whiteflies given a 24 h of inoculation access period ( IAP) to inoculate CCYV on cucumber plants showed a transmission efficiency rate of 72.73%. The retention site of CCYV virons was located in the foregut of virion-fed vectors. These results definitely indicated the semipersistent transmission mode of CCYV by B. tabaci MED.
作者：Li, Jingjing; Liang, Xiangzhi; Wang, Xueli; 等
作者单位：河南农业大学
期刊名称：SCIENTIFIC REPORTS
期刊影响因子：5.228
出版年份：2016
出版刊次：11
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