中文摘要：水稻条纹病毒（RSV）是东亚地区最严重的水稻病毒之一。为了探讨水稻如何对RSV侵染做出反应，作者通过深度测序整合了miRNA的表达和平行mRNA的转录谱。本文共鉴定了570个miRNAs，其中有69个miRNAs（56个上调和13个下调）明显因RSV侵染而改变。本研究发现的结果有助于理解病毒症状的发展以及RSV的致病机制。
外文摘要：Rice stripe virus (RSV) is one of the most serious rice viruses in East Asia. To investigate how rice responds to RSV infection, we integrated miRNA expression with parallel mRNA transcription profiling by deep sequencing. A total of 570 miRNAs were identified of which 69 miRNAs (56 up-regulated and 13 down-regulated) were significantly modified by RSV infection. Digital gene expression (DGE) analysis showed that 1274 mRNAs (431 up-regulated and 843 down-regulated genes) were differentially expressed as a result of RSV infection. The differential expression of selected miRNAs and mRNAs was confirmed by qRT-PCR. Gene ontology (GO) and pathway enrichment analysis showed that a complex set of miRNA and mRNA networks were selectively regulated by RSV infection. In particular, 63 differentially expressed miRNAs were found to be significantly and negatively correlated with 160 target mRNAs. Interestingly, 22 up-regulated miRNAs were negatively correlated with 24 down-regulated mRNAs encoding disease resistance-related proteins, indicating that the host defense responses were selectively suppressed by RSV infection. The suppression of both osa-miR1423-5p- and osa-miR1870-5p-mediated resistance pathways was further confirmed by qRT-PCR. Chloroplast functions were also targeted by RSV, especially the zeaxanthin cycle, which would affect the stability of thylakoid membranes and the biosynthesis of ABA. All these modifications may contribute to viral symptom development and provide new insights into the pathogenicity mechanisms of RSV.
作者：Yang, Jian; Zhang, Fen; Li, Jing; 等
作者单位：浙江农业科学院
期刊名称：PLOS ONE
期刊影响因子：3.057
出版年份：2016
出版刊次：1
点击下载：通过对microRNA组和转录组的综合分析来阐释易感水稻植株对水稻条纹叶枯病毒的反应