中文摘要：许多病毒由昆虫介体以持久性方式传播。持久性病毒在中肠上皮细胞中建立初始侵染，并从中肠细胞传播到中肠内脏肌肉。虽然小干扰RNA（siRNA）抗病毒路径可以调控昆虫介体体内的病毒传播，但是siRNA路径能否调控中肠上皮细胞的病毒传播仍然未知。南方水稻黑条矮缩病毒（SRBSDV）是一种仅由灰飞虱介体通过中肠上皮细胞传播的病毒。本研究结果表明，siRNA路径可以调控昆虫中肠上皮细胞的病毒初始侵染，从而影响病毒介体的传毒能力。
外文摘要：Numerous viruses are transmitted in a persistent manner by insect vectors. Persistent viruses establish their initial infection in the midgut epithelium, from where they disseminate to the midgut visceral muscles. Although propagation of viruses in insect vectors can be controlled by the small interfering RNA (siRNA) antiviral pathway, whether the siRNA pathway can control viral dissemination from the midgut epithelium is unknown. Infection by a rice virus (Southern rice black streaked dwarf virus [SRBSDV]) of its incompetent vector (the small brown planthopper [SBPH]) is restricted to the midgut epithelium. Here, we show that the siRNA pathway is triggered by SRBSDV infection in continuously cultured cells derived from the SBPH and in the midgut of the intact insect. Knockdown of the expression of the core component Dicer-2 of the siRNA pathway by RNA interference strongly increased the ability of SRBSDV to propagate in continuously cultured SBPH cells and in the midgut epithelium, allowing viral titers in the midgut epithelium to reach the threshold (1.99 x 10(9) copies of the SRBSDV P10 gene/mu g of midgut RNA) needed for viral dissemination into the SBPH midgut muscles. Our results thus represent the first elucidation of the threshold for viral dissemination from the insect midgut epithelium. Silencing of Dicer-2 further facilitated the transmission of SRBSDV into rice plants by SBPHs. Taken together, our results reveal the new finding that the siRNA pathway can control the initial infection of the insect midgut epithelium by a virus, which finally affects the competence of the virus's vector.
作者：Lan, Hanhong; Chen, Hongyan; Liu, Yuyan; 等
作者单位：福建农林大学
期刊名称：JOURNAL OF VIROLOGY
期刊影响因子：4.606
出版年份：2016
出版刊次：1
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