中文摘要：在植物病毒中，黄瓜花叶病毒（CMV）具有最广泛的寄主范围，可对农业造成巨大损失。本文通过研究发现， cmv1抗性等位基因（相当于将病毒从维管束鞘细胞传播至韧皮部细胞的守门人）可通过病毒株和细胞类型特异性方式制约病毒的系统感染。嫁接接种试验结果显示，cmv1还起着韧皮部内CMV的传播或将CMV从韧皮部传播出去的作用。这种新的以限制病毒系统性活动为特点的隐性抗性形式，开辟了设计可替代的甜瓜育种策略的可能性。
外文摘要：Cucumber mosaic virus (CMV) has the broadest host range among plant viruses, causing enormous losses in agriculture. In melon, strains of subgroup II are unable to establish a systemic infection in the near-isogenic line SC12-1-99, which carries the recessive resistance gene cmv1 from the accession PI161375, cultivar Songwhan Charmi'. Strains of subgroup I overcome cmv1 resistance in a manner dependent on the movement protein. We characterized the resistance conferred by cmv1 and established that CMV-LS (subgroup II) can move from cell to cell up to the veins in the inoculated leaf, but cannot enter the phloem. Immunogold labelling at transmission electron microscopy level showed that CMV-LS remains restricted to the bundle sheath (BS) cells in the resistant line, and does not invade vascular parenchyma or intermediary cells, whereas, in the susceptible line Piel de Sapo' (PS), the virus invades all vein cell types. These observations indicate that the resistant allele of cmv1 restricts systemic infection in a virus strain- and cell type-specific manner by acting as an important gatekeeper for virus progression from BS cells to phloem cells. Graft inoculation experiments showed that CMV-LS cannot move from the infected PS stock into the resistant cmv1 scion, thus suggesting an additional role for cmv1 related to CMV transport within or exit from the phloem. The characterization of this new form of recessive resistance, based on a restriction of virus systemic movement, opens up the possibility to design alternative approaches for breeding strategies in melon.
外文关键词：cmv1；Cucumber mosaic virus；host factor；phloem entry；recessive resistance； systemic infection；virus movement
作者：Guiu-Aragones, Celia；Amelia Sanchez-Pina, Maria；Antonio Diaz-Pendon, Juan；等
作者单位：CSIC IRTA UAB UB
期刊名称：MOLECULAR PLANT PATHOLOGY
期刊影响因子：4.335
出版年份：2016
出版刊次：8
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