中文摘要：RNA沉默信号在烟草花叶病毒增殖所必需的NtTOM1和NtTOM3中是沉默的，本文研究了RNA沉默信号在嫁接到烟草Sd1砧木上的非转基因番茄和烟草接穗中的传输。研究结果表明RNA沉默可传输至嫁接到Sd1砧木上的非转基因番茄和烟草接穗中，并且可在接穗中诱导抗病毒性。
外文摘要：We examined the transmission of RNA silencing signal in non-transgenic tomato and tobacco scions grafted onto the tobacco Sd1 rootstocks, which is silenced in both NtTOM1 and NtTOM3 required for tobamovirus multiplication. When the non-transgenic tomato scions were grafted onto the Sd1 rootstocks, RT-PCR analysis of the scions showed the reduced level of mRNA compared with that before grafting in both LeTH3 and LeTH1, tomato homologs of NtTOM1 and NtTOM3, respectively. siRNAs from both genes were detected in the scions after grafting but not before grafting. Further tomato scions were inoculated with Tomato mosaic virus (ToMV) and used for virus infection. They showed very low level of virus accumulation. Necrotic responding tobacco to tobamovirus was grafted onto the rootstock of Sdl. RT-PCR analysis showed low level expression of both NtTOM1 and NtTOM3 in the scions but siRNA was detected after grafting. When the leaves of scions were inoculated with ToMV or Tobacco mosaic virus, they produced very few local necrotic lesions (LNLs) while the control scions did many LNLs. These results suggest that RNA silencing was transmitted to non-transgenic tomato and tobacco scions after grafting onto the Sd1 rootstocks and that virus resistance was induced in the scions.
外文关键词：RNA silencing；Graft-transmission；Tobamovirus；Virus resistance；Tobacco；Tomato；TOM1/TOM3
作者：Ali, Md. Emran；Waliullah, Sumyya；Kobayashi, Kappei；等
作者单位：日本爱媛大学
期刊名称：JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY
期刊影响因子：1.352
出版年份：2016
出版刊次：7
点击下载：通过嫁接将RNA沉默信号从转基因沉默的烟草砧木传播至非转基因番茄和烟草接穗上，从而使其获得抗病毒性