中文摘要：黄瓜花叶病毒（CMV）可侵染多种寄主。由于缺少抗CMV的番茄品种，RNA干扰（RNAi）可被用于快速、有效生产转基因抗性番茄品种。本研究构建了针对CMV五个基因的五个植物内含子发夹结构RNA（ihpRNA）表达载体。通过农杆菌介导转化法获得携带表达载体的转基因番茄。将转基因植株高抗代当做砧木嫁接到非转基因番茄上，从而将抗性成功地转移到接穗上。利用一种新的植物扦插的方法进行砧木繁殖，获得了大量的抗病材料。此外，这种方法产生的接穗可保留检测不到的转基因抗性标记基因，这为规避公众对转基因生物（GMO）生物安全性的忧虑提供了新的途径。
外文摘要：Cucumber mosaic virus (CMV) can infect a wide range of host species. For the lacking of CMV resistant varieties of tomato, RNA interference (RNAi) can be used as a fast and effective method for the generation of transgenic resistant varieties. In this current study, five intron-spliced hairpin RNA (ihpRNA) plant expression vectors aimed at five genes of CMV have been constructed. Transgenic tomatoes were obtained by Agrobacterium tumefaciens-mediated transformation with expression vectors. Highly resistant generations of transgenic plants were employed as rootstocks and grafted onto non-transgenic tomatoes that resulted in the successful transfer of resistance to the scions. Using a novel method of plant cuttings for rootstock propagation, we obtained large quantities of disease-resistant material. Further, this method produces scions that can remain undetectable for transgenic resistance marker genes that may provide novel approaches to evade collective concerns about genetically-modified organism (GMO) biosafety.
外文关键词：Cucumber mosaic virus (CMV)；graft；RNA silencing；Solanum lycopersicum；virus resistance
作者：Bai Miao；Chen Wen-ting；Xie Bing-yan；等
作者单位：湖南农业大学
期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE
期刊影响因子：0.724
出版年份：2016
出版刊次：9
点击下载：一种通过嫁接转基因砧木提高番茄对黄瓜花叶病毒抗性的新策略