一种通过嫁接转基因砧木提高番茄对黄瓜花叶病毒抗性的新策略

A novel strategy to enhance resistance to Cucumber mosaic virus in tomato by grafting to transgenic rootstocks

点击次数:398   下载次数:228
中文摘要:黄瓜花叶病毒(CMV)可侵染多种寄主。由于缺少抗CMV的番茄品种,RNA干扰(RNAi)可被用于快速、有效生产转基因抗性番茄品种。本研究构建了针对CMV五个基因的五个植物内含子发夹结构RNA(ihpRNA)表达载体。通过农杆菌介导转化法获得携带表达载体的转基因番茄。将转基因植株高抗代当做砧木嫁接到非转基因番茄上,从而将抗性成功地转移到接穗上。利用一种新的植物扦插的方法进行砧木繁殖,获得了大量的抗病材料。此外,这种方法产生的接穗可保留检测不到的转基因抗性标记基因,这为规避公众对转基因生物(GMO)生物安全性的忧虑提供了新的途径。
外文摘要:Cucumber mosaic virus (CMV) can infect a wide range of host species. For the lacking of CMV resistant varieties of tomato, RNA interference (RNAi) can be used as a fast and effective method for the generation of transgenic resistant varieties. In this current study, five intron-spliced hairpin RNA (ihpRNA) plant expression vectors aimed at five genes of CMV have been constructed. Transgenic tomatoes were obtained by Agrobacterium tumefaciens-mediated transformation with expression vectors. Highly resistant generations of transgenic plants were employed as rootstocks and grafted onto non-transgenic tomatoes that resulted in the successful transfer of resistance to the scions. Using a novel method of plant cuttings for rootstock propagation, we obtained large quantities of disease-resistant material. Further, this method produces scions that can remain undetectable for transgenic resistance marker genes that may provide novel approaches to evade collective concerns about genetically-modified organism (GMO) biosafety.
外文关键词:Cucumber mosaic virus (CMV);graft;RNA silencing;Solanum lycopersicum;virus resistance
作者:Bai Miao;Chen Wen-ting;Xie Bing-yan;等
作者单位:湖南农业大学
期刊名称:JOURNAL OF INTEGRATIVE AGRICULTURE
期刊影响因子:0.724
出版年份:2016
出版刊次:9
点击下载:一种通过嫁接转基因砧木提高番茄对黄瓜花叶病毒抗性的新策略
  1. 编译服务:植物病毒学
  2. 编译者:戴红君
  3. 编译时间:2017-01-17