中文摘要：蓟马传播的番茄斑萎病毒是重要的经济性病毒，影响着全球大范围的农田和园艺作物。番茄斑萎病毒（TSWV）是番茄斑萎病毒属成员之一，其寄主范围广泛，有900多种寄主植物。这些病毒和寄主植物及昆虫介体之间通过RNAi途径的交互作用可能是致病的关键因素。本文首次研究比较了有或无TSWV发生的情况下寄主植物与昆虫介体之间小RNAs的生物合成。研究发现的结果有助于更好地制定作物与介体的具体控制策略。
外文摘要：Thrips-transmitted tospoviruses are economically important viruses affecting a wide range of field and horticultural crops worldwide. Tomato spotted wilt virus (TSWV) is the type member of the Tospovirus genus with a broad host range of more than 900 plant species. Interactions between these viruses and their plant hosts and insect vectors via RNAi pathways are likely a key determinant of pathogenicity. The current investigation, for the first time, compares biogenesis of small RNAs between the plant host and insect vector in the presence or absence of TSWV. Unique viral small interfering RNA (vsiRNA) profiles are evident for Arachis hypogaea (peanut) and Frankliniella fusca (thrips vector) following infection with TSWV. Differences between vsiRNA profiles for these plant and insect species, such as the relative abundance of 21 and 22 nt vsiRNAs and locations of alignment hotspots, reflect the diverse siRNA biosynthesis pathways of their respective kingdoms. The presence of unique vsiRNAs in F fusca samples indicates that vsiRNA generation takes place within the thrips, and not solely through uptake via feeding on vsiRNAs produced in infected A. hypogaea. The study also shows key vsiRNA profile differences for TSWV among plant families, which are evident in the case of A. hypogaea, a legume, and members of Solanaceae (S. lycopersicum and Nicotiana benthamiana). Distinctively, overall small RNA (sRNA) biogenesis in A. hypogaea is markedly affected with an absence of the 24 nt sRNAs in TSWV-infected plants, possibly leading to wide-spread molecular and phenotypic perturbations specific to this species. These findings add significant information on the host virus vector interaction in terms of RNAi pathways and may lead to better crop and vector specific control strategies.
外文关键词：tomato spotted wilt virus；Arachis hypogea；Frankliniella fusca；plant defense；plant-insect-virus interactions；vsiRNA；small RNA
作者：Fletcher, Stephen J.；Shrestha, Anita；Peters, Jonathan R.；等
作者单位：昆士兰大学
期刊名称：FRONTIERS IN PLANT SCIENCE
期刊影响因子：4.495
出版年份：2016
出版刊次：9
点击下载：番茄斑萎病毒基因组在寄主植物花生及其昆虫介体西花蓟马中的差异处理