中文摘要：感染水稻黑条矮缩病毒（RBSDV)）可导致玉米严重减产。为了了解玉米中RBSDV的响应路径，作者进行了小RNA组、降解组和转录组序列分析。玉米粗缩病（MRDD）可导致全球玉米严重减产，尤其是在中国夏玉米种植区，而MRDD是由RBSDV引起的。本文通过小RNA组、降解组和转录组序列分析来阐明玉米中RBSDV的响应路径。
外文摘要：Infection by Rice black-streaked dwarf virus (RBSDV) causes heavy yield losses in maize. We conducted analyses of microRNAs, the degradome, and transcriptome sequences in order to gain insights into RBSDV-responsive pathway(s) in maize. Maize rough dwarf disease (MRDD) is a viral infection that results in heavy yield losses in maize worldwide, particularly in the summer maize-growing regions of China. MRDD is caused by the Rice black-streaked dwarf virus (RBSDV). In the present study, analyses of microRNAs (miRNAs), the degradome, and transcriptome sequences were used to elucidate the RBSDV-responsive pathway(s) in maize. Genomic analysis indicated that the expression of three non-conserved and 28 conserved miRNAs, representing 17 known miRNA families and 14 novel miRNAs, were significantly altered in response to RBSDV when maize was inoculated at the V3 (third leaf) stage. A total of 99 target transcripts from 48 genes of 10 known miRNAs were found to be responsive to RBSDV infection. The annotations of these target genes include a SQUAMOSA promoter binding (SPB) protein, a P450 reductase, an oxidoreductase, and a ubiquitin-related gene, among others. Characterization of the entire transcriptome suggested that a total of 28 and 1085 differentially expressed genes (DEGs) were detected at 1.5 and 3.0 d, respectively, after artificial inoculation with RBSDV. The expression patterns of cell wall- and chloroplast-related genes, and disease resistance- and stress-related genes changed significantly in response to RBSDV infection. The negatively regulated genes GRMZM2G069316 and GRMZM2G031169, which are the target genes for miR169i-p5 and miR8155, were identified as a nucleolin and a NAD(P)-binding Rossmann-fold superfamily protein in maize, respectively. The gene ontology term GO:0003824, including GRMZM2G031169 and other 51 DEGs, was designated as responsive to RBSDV.
外文关键词：Degradome；maize；miRNA；Rice black-streaked dwarf virus；transcriptome；virus-response
作者：Zhou, Yu；Xu, Zhennan；Duan, Canxing；等
作者单位：中国农业科学院
期刊名称：JOURNAL OF EXPERIMENTAL BOTANY
期刊影响因子：5.677
出版年份：2016
出版刊次：8
点击下载：玉米中水稻黑条矮缩病毒响应的双重转录组分析