中文摘要：在南亚和东南亚地区，严重的水稻减产都是由水稻东格鲁病（RTD）引起的，这种病害由水稻东格鲁杆状病毒（RTBV）和水稻东格鲁球状病毒（RTSV）混合感染导致。为了开发基于转基因的RTBV抗性，利用RTBV的一个基因即ORF IV使容易转化的水稻品种Pusa Basmati-1产生基于RNA干扰的转基因抗性，随后通过转基因标记辅助选择法将该转基因导入到水稻品种ASD16中，ASD16在印度南部很受欢迎。本文研究报道了BC3F4和BC3F5代水稻植株对RTBV的抗性以及温室条件下的农艺性状。
外文摘要：Severe losses of rice yield in south and southeast Asia are caused by Rice tungro disease (RTD) induced by mixed infection of Rice tungro bacilliform virus (RTBV) and Rice tungro spherical virus (RTSV). In order to develop transgene-based resistance against RTBV, one of its genes, ORF IV, was used to generate transgenic resistance based on RNA-interference in the easily transformed rice variety Pusa Basmati-1, and the transgene was subsequently introgressed to rice variety ASD 16, a variety popular in southern India, using transgene marker-assisted selection. Here, we report the evaluation of BC3F4 and BC3F5 generation rice plants for resistance to RTBV as well as for agronomic traits under glasshouse conditions. The BC3F4 and BC3F5 generation rice plants tested showed variable levels of resistance, which was manifested by an average of twofold amelioration in height reduction, 1.5-fold decrease in the reduction in chlorophyll content, and 100- to 10,000-fold reduction in the titers of RTBV, but no reduction of RTSV titers, in three backcrossed lines when compared with the ASD 16 parent. Agronomic traits of some of the backcrossed lines recorded substantial improvements when compared with the ASD 16 parental line after inoculation by RTBV and RTSV. This work represents an important step in transferring RTD resistance to a susceptible popular rice variety, hence enhancing its yield in areas threatened by the disease.
外文关键词：Rice tungro disease；Real-time PCR；Resistance；Transgenic；Agronomic characters；Backcrossing
作者：Valarmathi, P.；Kumar, G.；Robin, S.；等
作者单位：Univ Delhi
期刊名称：VIRUS GENES
期刊影响因子：1.285
出版年份：2016
出版刊次：8
点击下载：通过回交育种转移抗水稻东格鲁病毒转基因后的水稻品种ASD 16的抗病毒性及农艺性状评价