中文摘要：葡萄阿尔及利亚潜隐病毒（GALV）是番茄丛矮病毒科番茄丛矮病毒属成员之一，不仅影响多年生木本植物葡萄，也影响草本植物烟草。本研究开发了烟草中基于GALV的基因表达和病毒诱导的基因沉默（VIGS）载体。研究结果表明，源自GALV的载体有望成为葡萄和烟草中有用的基因表达和VIGS载体。
外文摘要：Grapevine Algerian latent virus (GALV) is a member of the genus Tombusvirus in the Tombusviridae and infects not only woody perennial grapevine plant but also herbaceous Nicotiana benthamiana plant. In this study, we developed GALV-based gene expression and virus-induced gene silencing (VIGS) vectors in N. benthamiana. The GALV coat protein deletion vector, pGMG, was applied to express the reporter gene, green fluorescence protein (GFP), but the expression of GFP was not detected due to the necrotic cell death on the infiltrated leaves. The p19 silencing suppressor of GALV was engineered to inactivate its expression and GFP was successfully expressed with unrelated silencing suppressor, HC-Pro, from soybean mosaic virus. The pGMG vector was used to knock down magnesium chelatase (ChIH) gene in N. benthamaina and the silencing phenotype was clearly observed on systemic leaves. Altogether, the GALV-derived vector is expected to be an attractive tool for useful gene expression and VIGS vectors in grapevine as well as N. benthamiana.
外文关键词：Grapevine Algerian latent virus；virus-based expression vector；virus-induced gene silencing
作者：Park, Sang-Ho；Choi, Hoseong；Kim, Semin；等
作者单位：首尔大学
期刊名称：Plant Pathology Journal
期刊影响因子：0.92
出版年份：2016
出版刊次：8
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