中文摘要：病毒与植物沉默及与天然免疫途径之间的交互作用可能会改变感染病毒植株对非病毒病原体继发感染的敏感性。本研究发现，花椰菜花叶病毒（CaMV）P6是一个多功能的病毒效应器，可抑制沉默及天然免疫。P6介导的对氧化迸发和依赖于水杨酸的自噬的抑制可能会导致感染CaMV的植株易受到细菌侵染。
外文摘要：Virus interactions with plant silencing and innate immunity pathways can potentially alter the susceptibility of virus-infected plants to secondary infections with nonviral pathogens. We found that Arabidopsis plants infected with Cauliflower mosaic virus (CaMV) or transgenic for CaMV silencing suppressor P6 exhibit increased susceptibility to Pseudomonas syringae pv. tomato (Pst) and allow robust growth of the Pst mutant hrcC-, which cannot deploy effectors to suppress innate immunity. The impaired antibacterial defense correlated with the suppressed oxidative burst, reduced accumulation of the defense hormone salicylic acid (SA) and diminished SA-dependent autophagy. The viral protein domain required for suppression of these plant defense responses is dispensable for silencing suppression but essential for binding and activation of the plant target-of-rapamycin (TOR) kinase which, in its active state, blocks cellular autophagy and promotes CaMV translation. Our findings imply that CaMV P6 is a versatile viral effector suppressing both silencing and innate immunity. P6-mediated suppression of oxidative burst and SA-dependent autophagy may predispose CaMV-infected plants to bacterial infection.
外文关键词：autophagy；Cauliflower mosaic virus；effector protein；innate immunity；oxidative burst；RNA silencing；salicylic acid (SA)；target-of-rapamycin
作者：Zvereva, Anna S.；Golyaev, Victor；Turco, Silvia；等
作者单位：巴塞尔大学
期刊名称：NEW PHYTOLOGIST
期刊影响因子：7.21
出版年份：2016
出版刊次：8
点击下载：病毒蛋白抑制氧化迸发和依赖于水杨酸的自噬，并促进感染病毒植株上的细菌生长