中文摘要：辣椒褪绿病毒（CaCV）是澳大利亚和东南亚地区辣椒、番茄和花生作物上新兴的一种病原体。尚未有携带CaCV抗性的商业辣椒品种，但是在黄灯笼辣椒上鉴定出来的CaCV抗性正被导入到商业贝尔辣椒中。然而，众人在导致对CaCV侵染产生抗性反应的分子机制方面仍知之甚少。因此，转录组和表达分析数据为更好地理解CaCV抗性机制提供了重要的资源。本文将Illumina HiSeq平台与基于标签的数字基因表达系统相结合，组建了辣椒转录组并分析了基因表达。本研究获得的DE转录组分析数据为理解潜在的CaCV抗性机制提供了全面的基因表达信息
外文摘要：Background: Capsicum chlorosis virus (CaCV) is an emerging pathogen of capsicum, tomato and peanut crops in Australia and South-East Asia. Commercial capsicum cultivars with CaCV resistance are not yet available, but CaCV resistance identified in Capsicum chinense is being introgressed into commercial Bell capsicum. However, our knowledge of the molecular mechanisms leading to the resistance response to CaCV infection is limited. Therefore, transcriptome and expression profiling data provide an important resource to better understand CaCV resistance mechanisms. Methodology/Principal Findings: We assembled capsicum transcriptomes and analysed gene expression using Illumina HiSeq platform combined with a tag-based digital gene expression system. Total RNA extracted from CaCV/mock inoculated CaCV resistant (R) and susceptible (S) capsicum at the time point when R line showed a strong hypersensitive response to CaCV infection was used in transcriptome assembly. Gene expression profiles of R and S capsicum in CaCV-and buffer-inoculated conditions were compared. None of the genes were differentially expressed (DE) between R and S cultivars when mock-inoculated, while 2484 genes were DE when inoculated with CaCV. Functional classification revealed that the most highly up-regulated DE genes in R capsicum included pathogenesis-related genes, cell death-associated genes, genes associated with hormone-mediated signalling pathways and genes encoding enzymes involved in synthesis of defense-related secondary metabolites. We selected 15 genes to confirm DE expression levels by real-time quantitative PCR. Conclusion/Significance: DE transcript profiling data provided comprehensive gene expression information to gain an understanding of the underlying CaCV resistance mechanisms. Further, we identified candidate CaCV resistance genes in the CaCV-resistant C. annuum x C. chinense breeding line. This knowledge will be useful in future for fine mapping of the CaCV resistance locus and potential genetic engineering of resistance into CaCV-susceptible crops.
作者：Gamage, Shirani M. K. Widana；McGrath, Desmond J.；Persley, Denis M.；等
作者单位：昆士兰大学
期刊名称：PLOS ONE
期刊影响因子：3.057
出版年份：2016
出版刊次：7
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