中文摘要：烟粉虱是农作物上的主要害虫，以循环、持久的方式传播如番茄黄化曲叶病毒（TYLCV）类的双生病毒。转录组分析显示，B型烟粉虱knottin基因对各种胁迫都有响应。根据番茄双生病毒的摄取发现，4种knottin基因中有2种基因上调，即knot-1 （表达最高）和knot-3。本文研究了B型烟粉虱与TYLCV循环传播的相关性。研究发现，knot-1在限制昆虫可能摄取或传播的病毒数量上起着作用。因此可认为，knot-1可以通过限制病毒的总量（与烟粉虱介体有关）来保护B型烟粉虱对抗TYLCV导致的毒害影响。
外文摘要：The whitefly Bemisia tabaci is a major pest to agricultural crops. It transmits begomoviruses, such as Tomato yellow leaf curl virus (TYLCV), in a circular, persistent fashion. Transcriptome analyses revealed that B. tabaci knottin genes were responsive to various stresses. Upon ingestion of tomato begomoviruses, two of the four knottin genes were upregulated, knot-1 (with the highest expression) and knot-3. In this study, we examined the involvement of B. tabaci knottin genes in relation to TYLCV circulative transmission. Knottins were silenced by feeding whiteflies with knottin dsRNA via detached tomato leaves. Large amounts of knot-1 transcripts were present in the abdomen of whiteflies, an obligatory transit site of begomoviruses in their circulative transmission pathway; knot-1 silencing significantly depleted the abdomen from knot-1 transcripts. Knot-1 silencing led to an increase in the amounts of TYLCV ingested by the insects and transmitted to tomato test plants by several orders of magnitude. This effect was not observed following knot-3 silencing. Hence, knot-1 plays a role in restricting the quantity of virions an insect may acquire and transmit. We suggest that knot-1 protects B. tabaci against deleterious effects caused by TYLCV by limiting the amount of virus associated with the whitefly vector.
外文关键词：gene silencing; insect-plant-virus interaction; geminiviruses; knottin genes
作者：Shalev, Aliza Hariton；Sobol, Iris；Ghanim, Murad；等
作者单位：耶路撒冷希伯来大学
期刊名称：VIRUSES-BASEL
期刊影响因子：3.042
出版年份：2016
出版刊次：7
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