中文摘要：26S蛋白酶体亚基在植物与病原菌交互作用中的参与，以及每种亚基在独立调节许多细胞内和细胞间调控因子（可控制植物的生理与防御反应）活性中的作用都已有研究报道。本研究目的在于功能性描述番茄26S蛋白酶体亚基RPT4a (SlRPT4)基因，番茄耐性品种H-88-78-1感染新德里番茄曲叶病毒(ToLCNDV)后该基因会差异表达。分子分析表明，SlRPT4蛋白具有活跃的ATP酶活性。病毒诱导的SlRPT4基因基因沉默促进了H-88-78-1品种耐受性向敏感性的转换。
外文摘要：Involvement of 26S proteasomal subunits in plant pathogen-interactions, and the roles of each subunit in independently modulating the activity of many intra-and inter-cellular regulators controlling physiological and defense responses of a plant were well reported. In this regard, we aimed to functionally characterize a Solanum lycopersicum 26S proteasomal subunit RPT4a (SlRPT4) gene, which was differentially expressed after Tomato leaf curl New Delhi virus (ToLCNDV) infection in tolerant cultivar H-88-78-1. Molecular analysis revealed that SlRPT4 protein has an active ATPase activity. SlRPT4 could specifically bind to the stem-loop structure of intergenic region (IR), present in both DNA-A and DNA-B molecule of the bipartite viral genome. Lack of secondary structure in replication-associated gene fragment prevented formation of DNA-protein complex suggesting that binding of SlRPT4 with DNA is secondary structure specific. Interestingly, binding of SlRPT4 to IR inhibited the function of RNA Pol-II and subsequently reduced the bi-directional transcription of ToLCNDV genome. Virus-induced gene silencing of SlRPT4 gene incited conversion of tolerant attributes of cultivar H-88-78-1 into susceptibility. Furthermore, transient overexpression of SlRPT4 resulted in activation of programmed cell death and antioxidant enzymes system. Overall, present study highlights nonproteolytic function of SlRPT4 and their participation in defense pathway against virus infection in tomato.
作者：Sahu, Pranav Pankaj；Sharma, Namisha；Puranik, Swati；等
作者单位：印度新德里国家植物基因组研究所
期刊名称：SCIENTIFIC REPORTS
期刊影响因子：5.228
出版年份：2016
出版刊次：6
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