中文摘要：刺萼龙葵是一种“超级杂草”，生长快、分布广泛，且会产生对人类和其他动物有害的毒素茄碱。据悉，由于缺少刺萼龙葵相关的转基因方法和序列信息，尚未有针对其分子生物学方面的研究。病毒诱导的基因沉默（VIGS）是开展植物基因功能研究的有力工具；因此，本研究在刺萼龙葵中建立了源自烟草脆裂病毒（TRV）的VIGS。研究结果表明，VIGS技术和番茄基因组序列可被用于未来对刺萼龙葵基因功能的研究。
外文摘要：Solanum rostratum is a "super weed" that grows fast, is widespread, and produces the toxin solanine, which is harmful to both humans and other animals. To our knowledge, no study has focused on its molecular biology owing to the lack of available transgenic methods and sequence information for S. rostratum. Virus-induced gene silencing (VIGS) is a powerful tool for the study of gene function in plants; therefore, in the present study, we aimed to establish tobacco rattle virus (TRV)-derived VIGS in S. rostratum. The genes for phytoene desaturase (PDS) and Chlorophyll H subunit (ChlH) of magnesium protoporphyrin chelatase were cloned from S. rostratum and used as reporters of gene silencing. It was shown that high-efficiency VIGS can be achieved in the leaves, flowers, and fruit of S. rostratum. Moreover, based on our comparison of three different types of infection methods, true leaf infection was found to be more efficient than cotyledon and sprout infiltration in long-term VIGS in multiple plant organs. In conclusion, the VIGS technology and tomato genomic sequences can be used in the future to study gene function in S. rostratum.
作者：eng, Lan-Huan；Wang, Rui-Heng；Zhu, Ben-Zhong；等
作者单位：中国农业大学
期刊名称：PLOS ONE
期刊影响因子：3.057
出版年份：2016
出版刊次：6
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