中文摘要：本文报道了烟草环斑病毒（TRSV）载体在植物外源基因瞬时表达和内源基因功能分析方面的应用发展。将水母绿色荧光蛋白（GFP）基因嵌入到烟草环斑病毒运动蛋白（MP）和外壳蛋白（CP）间，可导致RNA2编码的病毒多蛋白的高水平表达。
外文摘要：We report here the development of tobacco ringspot virus (TRSV)-based vectors for the transient expression of foreign genes and for the analysis of endogenous gene function in plants using virus induced gene silencing. The jellyfish green fluorescent protein (GFP) gene was inserted between the TRSV movement protein (MP) and coat protein (CP) regions, resulting in high in-frame expression of the RNA2-encoded viral polyprotein. GFP was released from the polyprotein via an N-terminal homologous MP-CP cleavage site and a C-terminal foot-and-mouth disease virus (FMDV) 2 A catalytic peptide in Nicotiana benthamiana. The VIGS target gene was introduced in the sense and antisense orientations into a SnaBI site, which was created by mutating the sequence following the CP stop codon. VIGS of phytoene desaturase (PDS) in N. benthamiana, Arabidopsis ecotype Col-0, cucurbits and legumes led to obvious photo-bleaching phenotypes. A significant reduction in PDS mRNA levels in silenced plants was confirmed by semi-quantitative RT-PCR.
外文关键词：Tobacco ringspot virus；Foreign gene expression；Virus-induced gene silencing； Functional genomics；Phytoene desaturase；Nicotiana benthamiana；Arabidopsis；Legumes； Cucurbits
作者：Zhao, Fumei; Lim, Seungmo; Igori, Davaajargal; 等
作者单位：韩国科学技术联合大学院
期刊名称：VIROLOGY
期刊影响因子：3.2
出版年份：2016
出版刊次：5
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