中文摘要：玉米是一种重要的作物，其丰富的遗传多样性为遗传研究提供了先进的资源。然而，缺少一种可在大多数玉米品种中使用的快速瞬态基因功能分析的工具，从而导致了依赖于耗时的稳定转化和突变研究以获得答案。作者开发了一个用于玉米的基于自然玉米感染黄瓜花叶病毒（CMV）菌株即ZMBJ-CMV的高效病毒诱导的基因沉默（VIGS）载体。研究结果表明，研发的ZMBJ-CMV VIGS载体为快速有效的玉米基因功能研究提供了一种很好的工具。
外文摘要：Maize is a major crop whose rich genetic diversity provides an advanced resource for genetic research. However, a tool for rapid transient gene function analysis in maize that may be utilized in most maize cultivars has been lacking, resulting in reliance on time-consuming stable transformation and mutation studies to obtain answers. We developed an efficient virus-induced gene silencing (VIGS) vector for maize based on a naturally maize-infecting cucumber mosaic virus (CMV) strain, ZMBJ-CMV. An infectious clone of ZMBJ-CMV was constructed, and a vascular puncture inoculation method utilizing Agrobacterium was optimized to improve its utility for CMV infection of maize. ZMBJ-CMV was then modified to function as a VIGS vector. The ZMBJ-CMV vector induced mild to moderate symptoms in many maize lines, making it useful for gene function studies in critically important maize cultivars, such as the sequenced reference inbred line B73. Using this CMV VIGS system, expression of two endogenous genes, ZmPDS and ZmIspH, was found to be decreased by 75% and 78%, respectively, compared with non-silenced tissue. Inserts with lengths of 100-300 bp produced the most complete transcriptional and visual silencing phenotypes. Moreover, genes related to autophagy, ZmATG3 and ZmATG8a, were also silenced, and it was found that they function in leaf starch degradation. These results indicate that our ZMBJ-CMV VIGS vector provides a tool for rapid and efficient gene function studies in maize.
外文关键词：functional genomics study；maize inbred lines；knockdown；vascular puncture inoculation；cucumber mosaic virus；autophagy
作者：Wang, Rong；Yang, Xinxin；Wang, Nian；等
作者单位：中国农业大学
期刊名称：PLANT JOURNAL
期刊影响因子：5.468
出版年份：2016
出版刊次：4
点击下载：一种高效的病毒诱导的基因沉默载体在玉米功能基因组学研究中的应用