中文摘要：源自黄瓜绿斑驳花叶病毒（CGMMV）的小干扰RNAs（vsiRNAs）是烟草花叶病毒属成员之一，本文通过深度测序对黄瓜上该病毒的特征进行研究。研究发现，DCL2和DCL4可能参与了vsiRNAs的生成，这也是感染了CGMMV的黄瓜植株中一种常见的RNA沉默途径。本研究还能对某些vsiRNAs可能的靶标进行预测。
外文摘要：Virus-derived small interfering RNAs (vsiRNAs) of cucumber green mottle mosaic virus (CGMMV), a member of the genus Tobamovirus, were characterised in cucumber plants by deep sequencing. CGMMV vsiRNAs of 21-22 nt in length predominated, suggesting that there might be a conserved mechanism of DCL2 and DCL4 involvement in the biogenesis of vsiRNAs, as well as a common RNA silencing pathway in CGMMV-infected cucumber plants. The 5'-terminal base of vsiRNAs was biased towards C/A/U, suggesting that CGMMV vsiRNAs might be loaded into diverse AGO-containing RISCs to disturb the gene expression of host plants. Possible targets for some of the vsiRNAs were also predicted.
作者：Li, Yongqiang; Deng, Congliang; Shang, Qiaoxia;等
作者单位：北京农业大学
期刊名称：ARCHIVES OF VIROLOGY
期刊影响因子：2.255
出版年份：2016
出版刊次：2
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